ABSTRACT
Object This experiment was conducted to study the relationship between CFTR gene expression in the intestinal tissues and secretory diarrhea.Methods Twenty-four Kunming mice were selected, half male and half female, and were randomly divided into 3 groups ( n=8 in each group):control group with intraperitoneal injection of 0.2 mL nor-mal saline, and the experimental group of mice by intraperitoneal injection of lipopolysaccharide(LPS) (6 mg/kg· bw). The mental state and intestinal morphology of the mice at 1 h and 8 h after LPS injection were observed to assess whether the secretory diarrhea model was successfully established.The expression of CFTR gene segments of intestine tissue was de-tected by fluorescence quantitative PCR.Results LPS induced secretory diarrhea.CFTR gene was expressed in the mouse duodenum, jejunum, ileum and colon tissues with different expression abundance.It was highest in the colon, but the difference was not significant between intestinal segments.Compared with the control group, LPS up-regulated the tran-scription level of CFTR gene in the duodenum, jejunum and ileum, and down-regulated the transcription of CFTR gene in the colon.Conclusions The results of our study suggest that the changes of the transcriptional level of CFTR gene are closely related with the diarrhea induced by LPS and the effects in different intestinal segments on the diarrhea is different. The jejunum plays a crucial role and the colon plays a least role in the Cl-secretion.
ABSTRACT
Objective To investigate the clinical curative effect of tiotropium bromide combined with azithro -mycin in the treatment of elderly patients with chronic obstructive pulmonary disease ( COPD) and its effect on lung function.Methods 100 senile COPD patients were randomly divided into the two groups ,50 cases in each group.The control group was given tiotropium therapy , while the observation group used tiotropium bromide combined with azithromycin.The clinical efficacy and pulmonary function indices were compared between the two groups .Results Borg score and 6min walking distance (6MWD) of the observation group after treatment were (2.31 ±0.17) points and (380.13 ±15.07)m,which were significantly better than (3.17 ±0.26)points,(270.41 ±13.03)m of before treatment and (2.84 ±0.25)points,(320.18 ±14.78)m of the control group (t=6.84,8.56,7.90,8.73,7.79, 8.21,all P<0.05);FVC,FEV1 and FEV1/FVC of the observation group after treatment were (2.54 ±0.44) L, (2.13 ±0.35)L and (72.38 ±5.61)%,which were significantly higher than (1.63 ±0.37)L,(1.24 ±0.24)L and (47.30 ±5.48)%before treatment and (2.18 ±0.39) L,(1.69 ±0.35) L and (61.66 ±5.32)% of the control group (t=6.35,7.76,7.02,8.04,7.14,7.95,6.91,7.41,7.60,all P<0.05).Conclusion Tiotropium bromide combined with azithromycin has significant curative effect in the treatment of senile COPD and can obviously improve the pulmonary function of patients ,which has good clinical application value .